Confocal Microscopy

By Fatma Pri-Cakmak1; NACK Network2

1. Massachusetts Institute of Technology, Cambridge, MA 2. Nanotechnology Applications and Career Knowledge Network, Pennsylvania State University, University Park, PA

View Presentation

Category

Online Presentations

Published on

Bio

Fatma Pri-Cakmak

Fatma is a postdoctoral researcher in the laboratory of Dr. Ibrahim Cissé at MIT. Her research focuses on super-resolution imaging of condensates and viral RNA in live cells. Her research interests lie at the interface of physical chemistry, biophysics, and biology with a focus on understanding physicochemical properties of membrane-bound and membranelles organelles.

 

Sponsored by

Center for Nanotechnology Education and Utilization (CNEU)

Cite this work

Researchers should cite this work as follows:

  • Fatma Pri-Cakmak, NACK Network (2022), "Confocal Microscopy," https://nanohub.org/resources/36356.

    BibTex | EndNote

Time

Tags

  1. ATE Centers
  2. coacervate droplets
  3. coacervates
  4. coacervate systems
  5. Confocal Microscopy
  6. diffusion
  7. materials science
  8. microscopy
  9. NACK Network
  10. nano/bio
  11. photobleaching
Confocal Microscopy
  • Confocal Microscopy 1. Confocal Microscopy 0
    00:00/00:00
  • The Size of Things 2. The Size of Things 61.69502836169503
    00:00/00:00
  • History of Microscopes 3. History of Microscopes 123.52352352352352
    00:00/00:00
  • History of Microscopes 4. History of Microscopes 136.23623623623624
    00:00/00:00
  • History of Microscopes 5. History of Microscopes 180.88088088088088
    00:00/00:00
  • History of Microscopes 6. History of Microscopes 248.1147814481148
    00:00/00:00
  • Modern Microscope 7. Modern Microscope 321.02102102102106
    00:00/00:00
  • Modern Microscopy 8. Modern Microscopy 334.0006673340007
    00:00/00:00
  • Laser Scanning Confocal Microscopy 9. Laser Scanning Confocal Micros… 470.90423757090423
    00:00/00:00
  • Laser Scanning Confocal Microscopy 10. Laser Scanning Confocal Micros… 571.40473807140472
    00:00/00:00
  • Laser Scanning Confocal Microscopy 11. Laser Scanning Confocal Micros… 767.66766766766773
    00:00/00:00
  • Laser Scanning Confocal Microscopy 12. Laser Scanning Confocal Micros… 793.92726059392726
    00:00/00:00
  • Diffusion of Biomolecules in Vesicle Coated RNA-Based Coacervates 13. Diffusion of Biomolecules in V… 854.82148815482151
    00:00/00:00
  • Activities in cell can be compartmentalized by phase separation 14. Activities in cell can be comp… 882.08208208208214
    00:00/00:00
  • Diffusion of biomolecules indicates dynamic structure 15. Diffusion of biomolecules indi… 971.53820487153826
    00:00/00:00
  • PolyU/spermine coacervate system 16. PolyU/spermine coacervate syst… 1030.03003003003
    00:00/00:00
  • Partitioning in coacervate 17. Partitioning in coacervate 1104.4044044044044
    00:00/00:00
  • Concentration in the droplet can be calculated using microscopy 18. Concentration in the droplet c… 1131.9652986319654
    00:00/00:00
  • Partitioning in PolyU/spermine coacervate system 19. Partitioning in PolyU/spermine… 1199.1324657991324
    00:00/00:00
  • Partitioning in PolyU/spermine coacervate system 20. Partitioning in PolyU/spermine… 1305.2052052052052
    00:00/00:00
  • Partial Diffusion - What is Fluorescence Recovery After Photobleach (FRAP)? 21. Partial Diffusion - What is Fl… 1379.512846179513
    00:00/00:00
  • Entire Droplet Diffusion- FRAP 22. Entire Droplet Diffusion- FRAP 1459.8264931598264
    00:00/00:00
  • FRAP Curve and equations 23. FRAP Curve and equations 1529.8298298298298
    00:00/00:00
  • Partial droplet bleaching RNA recovery in polyU/spermine coacervate 24. Partial droplet bleaching RNA … 1550.9843176509844
    00:00/00:00
  • Partial droplet bleaching RNA recovery in polyU/spermine coacervate 25. Partial droplet bleaching RNA … 1570.7040373707041
    00:00/00:00
  • Entire droplet bleaching RNA recovery in polyU/spermine coacervate 26. Entire droplet bleaching RNA r… 1610.4771438104772
    00:00/00:00
  • Entire droplet bleaching RNA recovery in polyU/spermine coacervate 27. Entire droplet bleaching RNA r… 1625.2252252252254
    00:00/00:00
  • Vesicle assembly around coacervate droplets 28. Vesicle assembly around coacer… 1652.7861194527861
    00:00/00:00
  • Vesicle assembly around coacervate droplets 29. Vesicle assembly around coacer… 1676.0093426760095
    00:00/00:00
  • RNA diffusion in vesicle coated coacervate droplets 30. RNA diffusion in vesicle coate… 1706.3063063063064
    00:00/00:00
  • Conclusions 31. Conclusions 1742.3757090423758
    00:00/00:00
Pop Out