Oscillations in protein levels within cells help facilitate a variety of biological processes, from circadian rhythm to cell cycle progression. This research focuses on developing a platform for creating oscillatory circuits in mammalian cells by using several interrelated DNA constructs. The “activator” is one of these constructs and consists partly of a fusion between tetracycline transactivator (tTA) protein and green fluorescent protein (GFP) under the control of a tetracycline-response element (TRE) promoter. We aim to build this complex construct using molecular cloning methods and show that it can transiently carry out its intended function in mammalian cells prior to the integration of other circuit elements.

Rice University,  National Science Foundation (NSF) award #EEC-1406885

Researchers should cite this work as follows:

• Ariel Delos Reyes, Rice University (2022), "Building & Testing a Component of Synthetic Genetic Circuit in Mammalian Cells," https://nanohub.org/resources/36305.

  BibTex | EndNote

1. cell-cycle
2. cells
3. DNA
4. Mammalian Cells
5. nano
6. nano/bio