Fun Experiments You Can Only Do With Frogs
Fun Experiments You Can Only Do With Frogs
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1. Fun experiments you can ony do…
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2. The mitotic spindle
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3. The mitotic spindle
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4. The mitotic spindle
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5. The mitotic spindle
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6. How does the spindle scale to …
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7. Xenopus laevis egg extract
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8. Xenopus egg extract recapitula…
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9. Xenopus interspecies scaling
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10. Interspecies spindle scaling
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11. Microtubule destabilizing enzy…
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12. X. laevis and X.tropicalis
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13. Are spindle scaling mechanisms…
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14. Kif2a scales spindle size in H…
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15. Developmental Scaling
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16. Mitotic spindles scale with cy…
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17. Developmental scaling is also …
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18. Both volume and cytoplasmic co…
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19. Kif2a is differentially regula…
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20. Spindle size regulation by sen…
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21. Make physiological droplet bou…
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22. Prediction: Lipid composition …
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23. Importin α localization to th…
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24. Importin α is palmitoylated
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25. Is importin α localization to…
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26. Importin α localization to th…
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27. What about mitotic chromosome …
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28. How is DNA packaged into chrom…
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29. Strategy: high speed extract a…
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30. Optical trap assay
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31. Single DNA compaction in inter…
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32. What happens in the absence of…
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33. Core histone depletion alters …
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34. How do mitotic chromosomes sca…
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35. Developmental mitotic chromoso…
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36. Embryo chromosomes are 2-fold …
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37. Hi-C analysis
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38. Single chromosome immunofluore…
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39. Summary
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40. Outlook
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41. Acknoledgements
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