KIST/PU 2nd Annual Symposium: Molecular Imaging and Theragnosis
KIST/PU Ligand-functionalized gold nanorods as theragnostic agents
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- Presentation (with audio) (SWF)
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- Presentation Paper (PDF, 236 Kb)
| Contributor(s) | Alexander Wei Purdue University, West Lafayette |
|---|---|
| Abstract | Plasmon-resonant gold nanorods have been examined as multifunctional agents for imaging and photoactivated therapies. Nanorods can be imaged with single-particle sensitivity by two-photon luminescence (TPL) when excited by fs-pulsed laser irradiation, and have been monitored in vivo while passing through blood vessels at subpicomolar concentrations. TPL imaging can also be used to characterize the targeted delivery of ligand-functionalized nanorods to tumor cells. Nanorods were coated with oligoethyleneglycol (OEG) units using in situ dithiocarbamate formation, a novel and robust method of surface functionalization. Nanorods coated with methyl-terminated OEG chains were shielded from nonspecific cell uptake, whereas nanorods functionalized with folate-terminated OEG chains accumulated on the surface of tumor cells overexpressing their cognate receptor. Cells labeled with folate-functionalized nanorods became highly susceptible to photothermolysis when irradiated at plasmon resonance, often resulting in a dramatic blebbing of the cell membrane. The blebbing was determined not to be directly caused by nanorod-mediated photothermolysis, but rather due to the influx of extracellular Ca2+ following perforation of the cell membrane. |
| Biography |
Alexander Wei is an associate professor of chemistry in the College of Science at Purdue University. He received his Ph.D. in chemistry from Harvard University in 1995 and was a Fulbright and Chateaubriand Scholar at the Université Louis Pasteur in Strasbourg, France before starting his independent position at Purdue in 1997. He is an active member of the Purdue Cancer Center, the Birck Nanotechnology Center, the Bindley Biosciences Center, and the Oncological Sciences Center in the Discovery Park hub at Purdue University, and was recently appointed by the College of Science as a University Faculty Scholar.
Professor Wei’s research interests are rooted at the interface of organic chemistry and materials science, and their applications to biological systems. His interests include supramolecular surface science, nanoscale self-assembly, nanophotonics and nanomagnetism, cell-surface carbohydrates, and bionanotechnology. He has served on numerous federal and international peer review panels, and is the overseas editor for Science and Technology of Advanced Materials (Elsevier). |
| Credits | In conjunction with Terry B. Huff, Matthew N. Hansen, Ling Tong, Yan Zhao, Haifeng Wang, Daniel A. Zweifel, Ji-Xin Cheng. |
| Cite this work | If you reference this work in a publication, please cite as follows: |
| Date posted | 06 Dec, 2007 |
| Time | September 14, 2007 |
| Location | Burton Morgan Building, Room 121, Purdue University, West Lafayette, IN |
| Type | Online Presentations |
| Tags |
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Part of: KIST/PU 2nd Annual Symposium: Molecular Imaging and Theragnosis
KIST/PU 2nd Annual Symposium: Molecular Imaging and Theragnosis
This symposium presents an overview of the research of the Global Research Laboratory (GRL), a program that reflects a direct collaboration of scientific research between South Korea (Korea Institute of Science and Technology [KIST] and its affiliated research institutions) and the United States …
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Alexander Wei is an associate professor of chemistry in the College of Science at Purdue University. He received his Ph.D. in chemistry from Harvard University in 1995 and was a Fulbright and Chateaubriand Scholar at the Université Louis Pasteur in Strasbourg, France before starting his independent position at Purdue in 1997. He is an active member of the Purdue Cancer Center, the Birck Nanotechnology Center, the Bindley Biosciences Center, and the Oncological Sciences Center in the Discovery Park hub at Purdue University, and was recently appointed by the College of Science as a University Faculty Scholar.