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KIST/PU 2nd Annual Symposium: Molecular Imaging and Theragnosis

KIST/PU Multiplex Detection of Cell Surface Markers Using Plasmonic Sensors

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Contributor(s) Joseph M K Irudayaraj
Purdue University, West Lafayette
Abstract Gold nanorod molecular probes (GNrMPs) were designed and fabricated for multiplex identification of cell surface markers in HBECs. Cells were probed directly using dark field microscopy integrated with a spectral imager for simultaneous detection of up to three surface markers. The immunophenotype composition of these cell lines indicative of their metastasis potential was assessed using the GNrMPs. Due to the high multiplexing capacity of gold nanorods, the multiplexing capability of the GNrMP assay could possibly be expanded to 15 or greater. The technique has the potential to become an important tool for diagnosis and prognosis of breast and other cancers.
Biography Dr. Irudayaraj has a Bachelors degree in Engineering, a dual Masters respectively in Biosystems Engineering, and Computer Sciences from the University of Hawaii, and a Ph.D in Food and Bioprocess Engineering from Purdue University. He has held faculty positions at the University of Saskatchewan, Utah State University, and in the most immediate past, from The Pennsylvania State University. He has held adjoint positions at the Materials Research Institute at Penn State and the Dept of Applied and Engineering Physics in Cornell University.

Their team has developed a range of spectroscopic and biosensor technologies for disease diagnostics and food security monitoring. Tracking protein interactions and monitoring and detecting the dynamic state of single molecule events in cells is addressed through techniques such as Fluorescnece Correlation Spectroscopy, FRET, Fluorescence lifetime imaging, enhanced single cell Raman spectroscopy , Plasmonic sensing, and Optical Trap.

He has published over 130 refereed research articles in areas covering thermodynamics and viscoelactic modeling of biological systems, spectroscopic methods, bio and nanomaterial sensors, and is a member of American Chemical Society, Institute of Biological Engineering, Biophysical Society, and American Association for clinical Chemistry.
Credits In conjunction with: C. Yu, H. Nakshatri, S. Leavesley, B. Rajwa, and P. Robinson (Purdue University, West Lafayette, IN).

Partial funding from the Oncological Science Center and IUPUI Walter center grant is acknowledged. This work is also supported partially by Susan G. Komen Foundation grant BCTR0601111.
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  • Irudayaraj, Joseph M K (2007), "KIST/PU Multiplex Detection of Cell Surface Markers Using Plasmonic Sensors," http://www.nanohub.org/resources/3408/.

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Date posted 23 Oct, 2007
Time 03:45 PM, September 14, 2007
Location Burton Morgan Building, Room 121, Purdue University, West Lafayette, IN
Type Online Presentations
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  • 10.0 Ranking Workshops Part of: KIST/PU 2nd Annual Symposium: Molecular Imaging and Theragnosis

    KIST/PU 2nd Annual Symposium: Molecular Imaging and Theragnosis

    Type Workshops
    Date 23 Oct, 2007
    Avg. Rating 0.0 out of 5 stars  (0)
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    This symposium presents an overview of the research of the Global Research Laboratory (GRL), a program that reflects a direct collaboration of scientific research between South Korea (Korea Institute of Science and Technology [KIST] and its affiliated research institutions) and the United States …

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