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BME 695N Engineering Nanomedical Systems

BME 695N Lecture 8: Technologies for measuring nanomedical systems on/within cells

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Contributor(s) James Leary
Purdue University, West Lafayette
Abstract Outline:
  1. Introduction to measuring technologies for nanomedical system interaction with cells
    1. The importance of quantitative or at least semi-quantitative single cell measurements to detect presence and location of nanomedical systems
    2. Below "optical limit" imaging
    3. Requirements on the NMS to have X-ray dense, fluorescent, metallic, or magnetic cores
    4. Can you study living cells?
  2. Technologies – Advantages and disadvantages
    1. Flow cytometry – a "zero order" imaging device
    2. Scanning and Transmission electron microscopy
    3. Confocal microscopy – one and two-photon
    4. Surface Plasmon Resonance (SPR) Imaging
    5. Atomic Force Microscopy
    6. Magnetic Sorting/MRI contrast agents for in-vivo imaging
References
  • Liu, J. Scanning transmission electron microscopy and its application to the study of nanoparticles and nanoparticle systems. Journal of Electron Microscopy 54(3): 251–278 (2005).
  • Alschinger, M., Maniak, M., f. Stietz, F., Vartanyan, T., Trager. F. Application of metal nanoparticles in confocal laser scanning microscopy: improved resolution by optical field enhancement. Appl. Phys. B 76, 771–774, 2003.
  • Jain, P.K., El-Sayed, I.H., El-Sayed, M.A. Au nanoparticles target cancer. Nanotoday 2(1): 18-29, 2007.
  • Yoon, T-J, Yu, K.N., Kim, E., Kim, J.S., Kim, B.G., Yun, S-H, Sohn, B-Y, Cho, M-H, Lee,J-K, Park, S.B. Specific Targeting, Cell Sorting, and Bioimaging with Smart Magnetic Silica Core–Shell Nanomaterials. Small 2(2): 209 – 215, 2006.
  • Rao, A., Schoenenberger, M., Gnecco, E., Glatzel, Th, Meyer, E., Brändlin, D., Scandella, L. Characterization of nanoparticles using Atomic Force Microscopy. Journal of Physics: Conference Series 61 (2007) 971–976
  • Pris, A.D., Porter, M.D. Nanoparticle Coding: Size-Based Assays Using Atomic Force Microscopy. Langmuir 2004, 20, 6969-6973
Cite this work

If you reference this work in a publication, please cite as follows:

  • Leary, James (2007), "BME 695N Lecture 8: Technologies for measuring nanomedical systems on/within cells," http://www.nanohub.org/resources/3265/.

    BibTex | EndNote

Date posted 24 Sep, 2007
Time 04:30 PM, September 18, 2007
Location Biomedical Engineering Building, Room 1083
Type Online Presentations
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  • 8.9 Ranking Courses Part of: BME 695N Engineering Nanomedical Systems

    BME 695N Engineering Nanomedical Systems

    Type Courses
    Contributor(s) James Leary
    Date 28 Aug, 2007
    Avg. Rating 5.0 out of 5 stars  (1)
    Rate this

    This course will cover the basic concepts of design of integrated nanomedical systems for diagnostics and therapeutics. Topics to be covered include: why nanomedical approaches are needed, cell targeting strategies, choice of core nanomaterials, technologies for testing composition and structure …

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